e coli dh5α general cloning host strain lab stock l plantarum atcc8014  (ATCC)

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: One-step growth curve of phage Sa45lw using generic E. coli strain (ATCC 13706). The growth parameters of the phage indicate an eclipse period (EP) of 17 min, a latent period (LP) of 27 min, and average burst size (BS) of 80 phages per infected cell. Closed red triangles indicate non-chloroform-treated samples; closed blue circles indicate chloroform-treated samples. The error bars present the standard error of the mean (SEM) for each time point of the one-step growth curve.

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: Host range and efficiency of plating (EOP) of phage Sa45lw against various Shiga toxin-producing Escherichia coli (STEC) and Salmonella enterica strains

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques:

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: Antimicrobial activities of phage Sa45lw against (A) E. coli O45:H16 (RM13752) and (B) E. coli O157:H7 (RM35150) at an MOI of 10 in LB at 25°C for 24 h. The control group contained bacterial culture without phages (blue solid line), and the treatment group contained bacterial culture treated with phage Sa45lw (green dashed line). Data of each time point (0, 2, 4, 6 or 24 h) were analyzed separately, and asterisk indicates a significant difference at P < 0.05 between the control and treatment groups. The error bars present the SEM for each time point of the control and treatment.

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques: Control

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: Application of phage Sa45lw on the mung bean seeds contaminated with 4.8 log CFU/g of E. coli O45:H16 (RM13752) at an MOI of 1,000 for 15 min. Treated mung bean seeds were stored at room temperature (25°C) for 24 h. Asterisk indicates a significant difference at P < 0.05 between the time point (6 or 24 h) and the initial treatment (0 h). The error bars present the SEM for each time point.

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques:

Journal:

Article Title: Hemin Binding, Functional Expression, and Complementation Analysis of Pap 31 from Bartonella henselae

doi: 10.1128/JB.185.5.1739-1744.2003

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: All bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) Source or reference Strains Bartonella henselae Type strain ATCC 49882 E. coli K-12 EB53 aroB tsx malT hemA Generous gift of Klaus Hantke, Tübingen, Germany E. coli M15(pREP4) Host for expression vectors pQE70 and pQE60 Qiagen E. coli M15 pap31 As E. coli M15(pREP4) pRZn7-Pap31( ompT ) This study E. coli EB53(pREP4) As E. coli K-12 EB53 Km r lacI p15A ori This study E. coli EB53 pap31 As E. coli EB53(pREP4) pRZn7-Pap31( ompT ) Km r This study Plasmids pQE60 Expression vector; ColE1 PT5 lacO Amp r Qiagen pQE70 Expression vector; ColE1 PT5 lacO Amp r Qiagen pRZn7-Pap31 ( ompT ) As pQE70 pap31 ( ompT ) This study pREP4 lacI q Km r p14A ori Qiagen Open in a separate window Bacterial strains and plasmids used in this study

Techniques: Plasmid Preparation, Expressing

Journal:

Article Title: Hemin Binding, Functional Expression, and Complementation Analysis of Pap 31 from Bartonella henselae

doi: 10.1128/JB.185.5.1739-1744.2003

Figure Lengend Snippet: mRNA expression of pap31 in B. henselae (A) and of recombinant pap31 in E. coli M15(pREP4) (B). The amplified PCR fragment from B. henselae is 535 bp long, and that from E. coli is 291 bp long. MW, 100-bp molecular weight standard. (A) pap31 expression in B. henselae. Lane 1, RNA-PCR; lane 2, negative control; lane 3, positive control; lane 4, RT-PCR. (B) pap31 expression in E. coli. Lanes 1 to 4, RNA-PCR; lanes 5 to 8, RT-PCR. Lanes 1 and 5, E. coli M15(pREP4); lanes 2 and 6, E. coli M15(pREP4) with pQE70; lanes 3 and 7, E. coli M15 pap31 uninduced; lanes 4 and 8, E. coli M15 pap31 induced.

Article Snippet: All bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) Source or reference Strains Bartonella henselae Type strain ATCC 49882 E. coli K-12 EB53 aroB tsx malT hemA Generous gift of Klaus Hantke, Tübingen, Germany E. coli M15(pREP4) Host for expression vectors pQE70 and pQE60 Qiagen E. coli M15 pap31 As E. coli M15(pREP4) pRZn7-Pap31( ompT ) This study E. coli EB53(pREP4) As E. coli K-12 EB53 Km r lacI p15A ori This study E. coli EB53 pap31 As E. coli EB53(pREP4) pRZn7-Pap31( ompT ) Km r This study Plasmids pQE60 Expression vector; ColE1 PT5 lacO Amp r Qiagen pQE70 Expression vector; ColE1 PT5 lacO Amp r Qiagen pRZn7-Pap31 ( ompT ) As pQE70 pap31 ( ompT ) This study pREP4 lacI q Km r p14A ori Qiagen Open in a separate window Bacterial strains and plasmids used in this study

Techniques: Expressing, Recombinant, Amplification, Molecular Weight, Negative Control, Positive Control, Reverse Transcription Polymerase Chain Reaction

Journal:

Article Title: Hemin Binding, Functional Expression, and Complementation Analysis of Pap 31 from Bartonella henselae

doi: 10.1128/JB.185.5.1739-1744.2003

Figure Lengend Snippet: Western blot analysis of B. henselae whole-cell protein and recombinant Pap31 in E. coli M15(pREP4) with monoclonal Pap31 antibody VKS29. (A) Outer membrane proteins. M, prestained molecular mass standard (Bio-Rad Laboratories); lane 1, E. coli M15(pREP4); lane 2, E. coli M15(pREP4) with pQE70; lane 3, E. coli M15 pap31 before induction; lane 4, E. coli M15 pap31 after induction. The arrow indicates the recombinant Pap31. (B) Bartonella henselae whole-cell protein sample immunoblots incubated with monoclonal antibody VKS29 (lane 1) and polyclonal immunserum (lane 3). Lane 2, negative control with secondary antibody and without primary antibody (horseradish peroxidase-conjugated goat anti-mouse immunoglobulin G antibody).

Article Snippet: All bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) Source or reference Strains Bartonella henselae Type strain ATCC 49882 E. coli K-12 EB53 aroB tsx malT hemA Generous gift of Klaus Hantke, Tübingen, Germany E. coli M15(pREP4) Host for expression vectors pQE70 and pQE60 Qiagen E. coli M15 pap31 As E. coli M15(pREP4) pRZn7-Pap31( ompT ) This study E. coli EB53(pREP4) As E. coli K-12 EB53 Km r lacI p15A ori This study E. coli EB53 pap31 As E. coli EB53(pREP4) pRZn7-Pap31( ompT ) Km r This study Plasmids pQE60 Expression vector; ColE1 PT5 lacO Amp r Qiagen pQE70 Expression vector; ColE1 PT5 lacO Amp r Qiagen pRZn7-Pap31 ( ompT ) As pQE70 pap31 ( ompT ) This study pREP4 lacI q Km r p14A ori Qiagen Open in a separate window Bacterial strains and plasmids used in this study

Techniques: Western Blot, Recombinant, Membrane, Incubation, Negative Control

Journal:

Article Title: Hemin Binding, Functional Expression, and Complementation Analysis of Pap 31 from Bartonella henselae

doi: 10.1128/JB.185.5.1739-1744.2003

Figure Lengend Snippet: Two-dimensional analysis of the recombinant Pap31 with the outer membrane proteins from E. coli M15 pap31 before and after induction. Pap31 has an calculated pI of 5.2 and was detected with the monoclonal Pap31 antibody VKS29. Shown are outer membrane proteins from E. coli M15 pap31 before (A) and after (B) induction. The arrow points to the recombinant Pap31 at the pI of 5. A molecular mass standard (Bio-Rad Laboratories) is shown at the left side of each panel.

Article Snippet: All bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) Source or reference Strains Bartonella henselae Type strain ATCC 49882 E. coli K-12 EB53 aroB tsx malT hemA Generous gift of Klaus Hantke, Tübingen, Germany E. coli M15(pREP4) Host for expression vectors pQE70 and pQE60 Qiagen E. coli M15 pap31 As E. coli M15(pREP4) pRZn7-Pap31( ompT ) This study E. coli EB53(pREP4) As E. coli K-12 EB53 Km r lacI p15A ori This study E. coli EB53 pap31 As E. coli EB53(pREP4) pRZn7-Pap31( ompT ) Km r This study Plasmids pQE60 Expression vector; ColE1 PT5 lacO Amp r Qiagen pQE70 Expression vector; ColE1 PT5 lacO Amp r Qiagen pRZn7-Pap31 ( ompT ) As pQE70 pap31 ( ompT ) This study pREP4 lacI q Km r p14A ori Qiagen Open in a separate window Bacterial strains and plasmids used in this study

Techniques: Recombinant, Membrane